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Cancer Research
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利用卡尔瓦里亚共同培养和教条法测量骨重塑和再造肿瘤-骨微环境
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Cancer Research
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JoVE Journal
Cancer Research
Measuring Bone Remodeling and Recreating the Tumor-Bone Microenvironment Using Calvaria Co-culture and Histomorphometry
Please note that all translations are automatically generated.
Click here for the English version.
利用卡尔瓦里亚共同培养和教条法测量骨重塑和再造肿瘤-骨微环境
DOI:
10.3791/59028-v
•
08:24 min
•
March 14, 2020
•
Cinthia N. Cuero
,
Brenda Iduarte
,
Patricia Juárez
1
Biomedical Innovation Department
,
Center for Scientific Research and Higher Education at Ensenada
Chapters
00:00
Introduction
00:44
Calvarial Dissection
03:03
Calvaria and Cancer Cells Co-culture
04:19
Tissue Processing
05:49
Results
07:36
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
骨外植物
的外生培养
是骨生理学研究的宝贵工具，是骨改和骨病药物的潜在评价。提交的协议描述了从新生小鼠头骨分离的钙瓦里亚的制备和培养及其应用。
Tags
Bone Remodeling
Calvaria Co-culture
Tumor-bone Microenvironment
Histomorphometry
Ex Vivo Model
Mouse Calvaria
Organ Culture
Bone Regeneration
Cancer Cells
Bone-tumor Microenvironment
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