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应用先进的体外培养技术研究人肠道微生物群
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Bioengineering
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JoVE Journal Bioengineering
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
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应用先进的体外培养技术研究人肠道微生物群

DOI:
10.3791/59054-v

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06:23 min

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February 15, 2019

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, , , , ,
1Dairy and Functional Foods Research Unit, Eastern Regional Research Center,Agricultural Research Service, United States Department of Agriculture, 2ProDigest, 3Division of Gastroenterology, Hepatology, and Nutrition,Children’s Hospital of Philadelphia

Chapters

  • 00:04Title
  • 00:36System Inoculation
  • 01:38Luminal Fluid and Mucosal Sample Harvest
  • 03:30Daily Feeding Cycles
  • 04:27Results: Representative Stable Community Comparison Between the In Vitro System and the Fecal Inoculum
  • 05:52Conclusion

Summary

Automatic Translation

Automatic Translation

在这里, 我们提出了一个在体外培养结肠肠道微生物群的方案,使用一系列生物反应器, 模拟胃肠道的生理条件。

Tags

In Vitro CulturingGut MicrobiotaColonDefined MediumMucinFecal HomogenateBioreactorPH ControlLuminal SampleMucosal SampleDNA AnalysisShort-chain Fatty Acids

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