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Bioengineering
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体外養殖人間の腸内細菌を研究する技術を適用する高度な
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Applying Advanced In Vitro Culturing Technology to Study the Human Gut Microbiota
Please note that all translations are automatically generated.
Click here for the English version.
体外養殖人間の腸内細菌を研究する技術を適用する高度な
DOI:
10.3791/59054-v
•
06:23 min
•
February 15, 2019
•
Jenni Firrman
,
LinShu Liu
,
Pieter Van den Abbeele
,
Ceylan Tanes
,
Kyle Bittinger
,
Peggy Tomasula
1
Dairy and Functional Foods Research Unit, Eastern Regional Research Center
,
Agricultural Research Service, United States Department of Agriculture
,
2
ProDigest
,
3
Division of Gastroenterology, Hepatology, and Nutrition
,
Children’s Hospital of Philadelphia
Chapters
00:04
Title
00:36
System Inoculation
01:38
Luminal Fluid and Mucosal Sample Harvest
03:30
Daily Feeding Cycles
04:27
Results: Representative Stable Community Comparison Between the In Vitro System and the Fecal Inoculum
05:52
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
ここでは、胃腸管の生理的条件をシミュレートするバイオリアクターの一連を使用して体外のコロン
,
の腸内細菌を培養するためのプロトコルを提案する.
Tags
In Vitro Culturing
Gut Microbiota
Colon
Defined Medium
Mucin
Fecal Homogenate
Bioreactor
PH Control
Luminal Sample
Mucosal Sample
DNA Analysis
Short-chain Fatty Acids
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