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基于荧光的感染组织细菌基因调控方法
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Genetics
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JoVE Journal
Genetics
A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
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基于荧光的感染组织细菌基因调控方法
DOI:
10.3791/59055-v
•
07:10 min
•
February 19, 2019
•
Ranjan K. Behera
,
Kevin D. Mlynek
,
Matthew S. Linz
,
Shaun R. Brinsmade
1
Department of Biology
,
Georgetown University
Chapters
00:04
Title
00:38
Bacterial Inoculum Preparation and Infection
02:17
Organ Harvest and Tissue Processing
03:20
Laser Scanning Confocal Microscopy and Image Processing
04:52
Results: Representative Localization of
nuc
-sGFP in the Staphylococcal Abscess Microenvironment
06:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本文介绍了一种在细胞水平上分析动物组织中细菌基因表达的方法。该方法为研究细菌种群中发生的表型多样性提供了资源, 以应对感染过程中的组织环境。
Tags
Bacterial Gene Regulation
Fluorescence-based Method
Bacterial Gene Expression
S. Aureus
Hemolytic Phenotype
Bacterial Physiology
Antivirulence
Antibacterial Compounds
Cryosectioning
DAPI Staining
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