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Cancer Research
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酵母作为开发功能分析研究人类P53的机箱
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Cancer Research
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Please note that all translations are automatically generated.
Click here for the English version.
酵母作为开发功能分析研究人类P53的机箱
DOI:
10.3791/59071-v
•
14:57 min
•
August 04, 2019
•
Paola Monti
,
Bartolomeo Bosco
,
Sara Gomes
,
Lucilia Saraiva*
3
,
Gilberto Fronza*
1
,
Alberto Inga*
2
1
Mutagenesis and Cancer Prevention Unit
,
Ospedale Policlinico San Martino
,
2
Department CIBIO
,
University of Trento
,
3
LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy
,
University of Porto
Chapters
00:00
Title
00:54
Construction of
ADE2
or
LUC1
Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
03:06
Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based
ADE2
Yeast Assay
04:27
Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based
LUC1
Yeast Assay
07:06
Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
08:08
Results
13:51
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里提出的四个协议,以构造和利用酵母
糖糖酶
报告菌株,以研究人类P53转活化潜力,其各种癌症相关突变的影响,共同表达的相互作用蛋白,和特定小分子的影响。
Tags
Yeast
P53
Transcription
Reporter Gene
Adenine-2
Firefly Luciferase
Lithium Acetate
Transformation
PCR
Sanger Sequencing
Transactivation
Color-based Assay
Article
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