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酵母作为开发功能分析研究人类P53的机箱
JoVE Journal
Cancer Research
Author Produced
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JoVE Journal Cancer Research
Yeast As a Chassis for Developing Functional Assays to Study Human P53
DOI:

14:57 min

August 04, 2019

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Chapters

  • 00:00Title
  • 00:54Construction of ADE2 or LUC1 Reporter Yeast Strain that Contains a Specific RE (yAFM-RE or yLFM-RE)
  • 03:06Evaluation of P53 Protein Transactivation Ability using the Qualitative Color-based ADE2 Yeast Assay
  • 04:27Evaluation of P53 Protein Transactivation Ability using the Quantitative Luminescence-based LUC1 Yeast Assay
  • 07:06Evaluation of P53 Protein Growth Inhibition using the Yeast Phenotypic Assay
  • 08:08Results
  • 13:51Conclusion

Summary

Automatic Translation

这里提出的四个协议,以构造和利用酵母糖糖酶报告菌株,以研究人类P53转活化潜力,其各种癌症相关突变的影响,共同表达的相互作用蛋白,和特定小分子的影响。

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