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改进 x 射线激光时分辨序列飞秒晶体学微晶的高粘度挤出
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JoVE Journal Chemistry
Improving High Viscosity Extrusion of Microcrystals for Time-resolved Serial Femtosecond Crystallography at X-ray Lasers
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改进 x 射线激光时分辨序列飞秒晶体学微晶的高粘度挤出

DOI:
10.3791/59087-v

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07:26 min

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February 28, 2019

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, , , , , , , ,
1Division of Biology and Chemistry – Laboratory for Biomolecular Research,Paul Scherrer Institut, 2Photon Science Division – SwissFEL,Paul Scherrer Institut, 3RIKEN SPring-8 Center, 4Department of Cell Biology, Graduate School of Medicine,Kyoto University, 5Department of Biology,ETH Zürich

Chapters

  • 00:04Title
  • 00:25Protein Crystal Sample Preparation
  • 03:00Testing Sample Extrusion Using a High-Speed Camera Setup
  • 05:43Results: Extrusion of Protein Microcrystals from a High Viscosity Micro-extrusion Injector
  • 07:03Conclusion

Summary

Automatic Translation

Automatic Translation

时间解析的飞秒晶体学实验的成功取决于有效的样品传递。在这里, 我们描述了从高粘度微挤压喷射器中优化细菌视紫红质微晶挤出的方案。该方法依赖于样品同质化与一种新的三向耦合器和可视化与高速相机。

Tags

High Viscosity ExtrusionMicrocrystalsTime-resolved Serial Femtosecond CrystallographyX-ray LasersLCPMonooleinMAG 7.9Liquid ParaffinSyringeCubic PhaseExtrusion TestingHPLC Pump

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