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En roman menneskelige Epithelial Enteroid modell for nekrotiserende enterokolitt
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
DOI:

08:42 min

April 10, 2019

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Chapters

  • 00:04Title
  • 01:25Crypt Isolation and Plating from Whole Tissue
  • 05:21Induction of Experimental NEC and Preparation for Paraffin Embedding
  • 07:22Results: Analysis of Enteroids
  • 08:21Conclusion

Summary

Automatic Translation

Enteroids dukker opp som en ny modell i studiet av menneskelig sykdom. Protokollen beskriver hvordan å simulere en enteroid modell av menneskelig necrotizing enterokolitt bruker lipopolysakkarid (LPS) behandling av enteroids fra neonatal vev. Samlet enteroids viser inflammatoriske endringer som dem sett i menneskelig necrotizing enterokolitt.

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