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用于可重复模式形成的混合凝胶立方体器件中的初始三维单元群集控制

JoVE Journal
Bioengineering

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JoVE Journal Bioengineering

Initial 3D Cell Cluster Control in a Hybrid Gel Cube Device for Repeatable Pattern Formations

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用于可重复模式形成的混合凝胶立方体器件中的初始三维单元群集控制

DOI:

10.3791/59214-v

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05:22 min

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March 21, 2019

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Masaya Hagiwara, Rina Nobata, Tomohiro Kawahara

¹NanoSquare Research Institute,Osaka Prefecture University, ²Department of Bioscience and Informatics,Osaka Prefecture University, ³Department of Biological Functions Engineering,Kyushu Institute of Technology

Chapters

00:04Title
00:26Hybrid Gel Cube (HGC) Device Fabrication
01:14Hydrogel Initial Cell Cluster Shape Management
02:57Multi-Directional Imaging
04:09Results: Representative Multi-Dimensional Scanning of HGC-Cultured Cells
04:50Conclusion

Summary

Automatic Translation

我们提出了一个控制三维细胞外基质中初始细胞簇形状的过程, 以获得可重复的模式形成。采用含有两种不同水凝胶的立方装置, 实现组织形态形成的多向成像。

Tags

3D Cell Cluster Hybrid Gel Cube Repeatable Pattern Polycarbonate Cubic Frame Agarose Extracellular Matrix Cell Culture Micro Mold Cell Suspension Cell Culture Medium 24-well Plate

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