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体内自身免疫性脑髓炎实验性神经血管内障碍和胶质屏障的可视化
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
DOI:

10:50 min

March 26, 2019

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Chapters

  • 00:04Title
  • 00:53Immunization of C57BL/6 Mice
  • 02:04Scoring of EAE Mice
  • 03:08In Vivo Permeability Assay
  • 04:25Dissection and Freezing of Brains
  • 06:15In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
  • 08:21Results: Course of EAE and In Vivo Permeability
  • 09:42Conclusion

Summary

Automatic Translation

在这里, 我们提出的方案, 以调查损伤的神经血管单位在实验性自身免疫性脑髓炎在体内。我们专门讨论了如何确定血液脑屏障通透性和明胶酶活性与白细胞迁移跨越胶质细胞限制。

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