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Journal
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Immunology and Infection
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体内自身免疫性脑髓炎实验性神经血管内障碍和胶质屏障的可视化
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Immunology and Infection
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JoVE Journal
Immunology and Infection
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
Please note that all translations are automatically generated.
Click here for the English version.
体内自身免疫性脑髓炎实验性神经血管内障碍和胶质屏障的可视化
DOI:
10.3791/59249-v
•
10:50 min
•
March 26, 2019
•
Silvia M. Tietz
,
Britta Engelhardt
1
Theodor Kocher Institute
,
University of Bern
Chapters
00:04
Title
00:53
Immunization of C57BL/6 Mice
02:04
Scoring of EAE Mice
03:08
In Vivo Permeability Assay
04:25
Dissection and Freezing of Brains
06:15
In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
08:21
Results: Course of EAE and In Vivo Permeability
09:42
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里, 我们提出的方案, 以调查损伤的神经血管单位在实验性自身免疫性脑髓炎在体内。我们专门讨论了如何确定血液脑屏障通透性和明胶酶活性与白细胞迁移跨越胶质细胞限制。
Tags
Experimental Autoimmune Encephalomyelitis
Blood-brain Barrier
Matrix Metalloproteinases
Immune Cell Infiltration
Neuroinflammation
EAE Scoring
Mouse Model
MOG Peptide
Freund’s Adjuvant
Pertussis Toxin
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