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Visualisere værdiforringelse af endotel og Glial barrierer af neurovaskulære enhed under eksperimentelle Autoimmune Encephalomyelitis In Vivo
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
DOI:

10:50 min

March 26, 2019

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Chapters

  • 00:04Title
  • 00:53Immunization of C57BL/6 Mice
  • 02:04Scoring of EAE Mice
  • 03:08In Vivo Permeability Assay
  • 04:25Dissection and Freezing of Brains
  • 06:15In Situ Zymography Combined with Laminin/CD45 Immunofluorescence Staining
  • 08:21Results: Course of EAE and In Vivo Permeability
  • 09:42Conclusion

Summary

Automatic Translation

Vi præsenterer her, protokoller for at undersøge værdiforringelse af neurovaskulære enhed under eksperimentelle autoimmune encephalomyelitis in vivo. Vi behandle specifikt, hvordan du bestemme blod - hjerne barrieren permeabilitet og gelatinase aktivitet involveret i leukocyt migration på tværs af glia limitans.

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