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Biochemistry
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生物技术环境下的氟黏膜化人乳三糖--用基因编码生物传感器分析
JoVE Journal
Biochemistry
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JoVE Journal
Biochemistry
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
Please note that all translations are automatically generated.
Click here for the English version.
生物技术环境下的氟黏膜化人乳三糖--用基因编码生物传感器分析
DOI:
10.3791/59253-v
•
10:17 min
•
April 13, 2019
•
Fatima Enam
,
Thomas J. Mansell
1
Department of Chemical and Biological Engineering
,
Iowa State University
Chapters
00:04
Title
01:12
Cell Culture
01:53
Measurement of Fluorescence and Limits of Detection
02:46
Detection and Quantification of 2 -FL in a Producer Strain
04:58
Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
06:04
Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
06:48
Selective Removal of Lactose: Lactose Precipitation with Ethanol
07:41
Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
09:37
Conclusion
Summary
Automatic Translation
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Automatic Translation
本文介绍了利用全细胞生物传感器对岩藻糖类母乳低聚糖 (Hmo) 进行高通量检测和定量的研究。我们还在这里演示了这个平台的适应, 以分析 HMO 生产菌株, 重点是提高信噪比。
Tags
Human Milk Oligosaccharides
Fucosylated Trisaccharides
Biotechnological Production
Genetically Encoded Biosensors
High-throughput Screening
Carbohydrate Linkage Characterization
Infant Formula
Lactose Removal
Flow Cytometry
2’FL Biosensing
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