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Analyse av Fucosylated Human melk Trisaccharides i bioteknologisk sammenheng med genetisk kodet Biosensors
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors
DOI:

10:17 min

April 13, 2019

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Chapters

  • 00:04Title
  • 01:12Cell Culture
  • 01:53Measurement of Fluorescence and Limits of Detection
  • 02:46Detection and Quantification of 2 -FL in a Producer Strain
  • 04:58Selective Removal of Lactose: Treatment with Commercial Purified β-galactosidase
  • 06:04Selective Removal of Lactose: Pre-treatment with LacZ+ Strain
  • 06:48Selective Removal of Lactose: Lactose Precipitation with Ethanol
  • 07:41Results: Analysis of Fucosylated Human Milk Trisaccharides Using Genetically Encoded Biosensors
  • 09:37Conclusion

Summary

Automatic Translation

Vi beskriver her høy gjennomstrømming gjenkjenning og kvantifisering av fucosylated morsmelk oligosaccharides (HMOs) bruker en hel celle biosensor. Også viser vi her, tilpasning av denne plattformen mot analyse av HMO produksjon stammer, fokusere på å forbedre signal til støyforhold.

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