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线虫中耳炎群链球菌引起氧化应激的研究
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Developmental Biology
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JoVE Journal Developmental Biology
Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans
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线虫中耳炎群链球菌引起氧化应激的研究

DOI:
10.3791/59301-v

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09:36 min

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March 23, 2019

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, ,
1Department of Diagnostic and Biomedical Sciences, School of Dentistry,University of Texas Health Science Center

Chapters

  • 00:04Title
  • 00:29Preparation of THY (Todd-Hewitt Yeast Extract) Agar Plates and Mitis Group Streptococci
  • 02:12Survival Assays
  • 04:04Preparation of Agarose Pads for Microscopy
  • 04:52Observation of SKN-1 Localization in Response to Streptococcus Infection
  • 07:36Results: Killing of the Worms
  • 09:02Conclusion

Summary

Automatic Translation

Automatic Translation

线虫是解剖寄主-病原体相互作用的良好模型。这里描述的是一个协议, 感染蠕虫与炎组链球菌成员, 并确定激活的氧化应激反应对 h2o2 产生的这一组生物。

Tags

Oxidative StressMitis Group StreptococciCaenorhabditis ElegansHydrogen PeroxidePathogenicityStress ResponseReactive Oxygen SpeciesTodd Hewitt AgarTHY AgarCatalaseE. Coli OP-50NGM PlatesMicroaerophilic Environment

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