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At studere Oxidative Stress forårsaget af Mitis gruppe streptokokker i Caenorhabditis elegans
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Chapters
- 00:04Title
- 00:29Preparation of THY (Todd-Hewitt Yeast Extract) Agar Plates and Mitis Group Streptococci
- 02:12Survival Assays
- 04:04Preparation of Agarose Pads for Microscopy
- 04:52Observation of SKN-1 Localization in Response to Streptococcus Infection
- 07:36Results: Killing of the Worms
- 09:02Conclusion
Nematode Caenorhabditis elegans er en fremragende model for dissekere vært-patogen interaktioner. Beskrevet her er en protokol til at inficere orm med medlemmer af mitis gruppe streptokokker og bestemme aktivering af oxidative stress reaktion mod H2O2 produceret af denne gruppe af organismer.
