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JoVE Journal
Bioengineering
Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure
Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure

Material Formation of Recombinant Spider Silks through Aqueous Solvation using Heat and Pressure

Full Text
5,767 Views
10:26 min
May 6, 2019

DOI: 10.3791/59318-v

Justin A. Jones1, Thomas I. Harris1, Brianne E. Bell1, Paula F. Oliveira1

1Department of Biology,Utah State University

Here, we present a protocol to produce water soluble recombinant spider silk protein solutions and the material forms that can be formed from those solutions.

This method of solubilizing recombinant spider silks produces material forms that are not possible using traditional harsh organic solvents. Further, the process is grain, resulting in a simple protein solution in water. By utilizing this method, the materials formed or produced maintain the desirable characteristics of spider silk proteins.

This solubilization technique for recombinant spider silks allows for the solubilization of recombinant spider silks in a one step process. This is desirable because these notoriously difficult to produce proteins aren't lost through extensive processing. Further, since the solutions are just protein in water, other biologically active compounds can be added if that's desirable for the application.

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