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DOI: 10.3791/59318-v
Here, we present a protocol to produce water soluble recombinant spider silk protein solutions and the material forms that can be formed from those solutions.
This method of solubilizing recombinant spider silks produces material forms that are not possible using traditional harsh organic solvents. Further, the process is grain, resulting in a simple protein solution in water. By utilizing this method, the materials formed or produced maintain the desirable characteristics of spider silk proteins.
This solubilization technique for recombinant spider silks allows for the solubilization of recombinant spider silks in a one step process. This is desirable because these notoriously difficult to produce proteins aren't lost through extensive processing. Further, since the solutions are just protein in water, other biologically active compounds can be added if that's desirable for the application.
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