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JoVE Journal Neuroscience
Conversione di cellule staminali pluripotenti indotte dall'uomo (IPSC) in neuroni motore spinale e cranico funzionale utilizzando vettori PiggyBac
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Conversione di cellule staminali pluripotenti indotte dall'uomo (IPSC) in neuroni motore spinale e cranico funzionale utilizzando vettori PiggyBac

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Questo protocollo consente la conversione rapida ed efficiente delle cellule staminali pluripotenti indotte in neuroni motori con un'identità spinale o cranica, mediante l'espressione ectopica dei fattori di trascrizione dai vettori inducibili piggyBac.

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Neuroscienze problema 147 cellule staminali pluripotenti indotte motoneuroni piggyBac differenziazione fattori di trascrizione Phox2a Isl1 Ngn2 Lhx3
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