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Bioengineering
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构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Please note that all translations are automatically generated.
Click here for the English version.
构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南
DOI:
10.3791/59360-v
•
08:13 min
•
April 08, 2019
•
Jialei Tang
,
Chun-Hung Weng
,
Jeffrey B. Oleske
,
Kyu Young Han
1
CREOL, The College of Optics and Photonics
,
University of Central Florida
,
2
Andor Technology
,
Oxford Instruments
Chapters
00:04
Title
00:28
Preliminary Setup
01:11
Setting up the Detection Path
02:14
Setting up the Excitation Path and the Cylindrical Lenses
04:59
Testing Tile Imaging
05:53
Highly Inclined Swept Tile (HIST) Imaging
06:52
Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
07:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
详细介绍了如何建立高倾斜的扫描瓷砖 (HIST) 显微镜及其在单分子成像中的应用。
Tags
Single-molecule Imaging
Extended Field-of-view
Swept Tile Microscope
Piezo Stage
Collimated Light Source
Dichroic Mirror
Tube Lens
Multi-band Pass Filter
Fold-mirror
Objective Lens
Airy Disc
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