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高傾斜の構築ガイド拡張ビュー フィールドの単一分子イメージング用タイル顕微鏡を掃引
JoVE Journal
Bioengineering
This content is Free Access.
JoVE Journal
Bioengineering
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
Please note that all translations are automatically generated.
Click here for the English version.
高傾斜の構築ガイド拡張ビュー フィールドの単一分子イメージング用タイル顕微鏡を掃引
DOI:
10.3791/59360-v
•
08:13 min
•
April 08, 2019
•
Jialei Tang
,
Chun-Hung Weng
,
Jeffrey B. Oleske
,
Kyu Young Han
1
CREOL, The College of Optics and Photonics
,
University of Central Florida
,
2
Andor Technology
,
Oxford Instruments
Chapters
00:04
Title
00:28
Preliminary Setup
01:11
Setting up the Detection Path
02:14
Setting up the Excitation Path and the Cylindrical Lenses
04:59
Testing Tile Imaging
05:53
Highly Inclined Swept Tile (HIST) Imaging
06:52
Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
07:38
Conclusion
Summary
Automatic Translation
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Automatic Translation
詳細な指示は、タイル (HIST) 顕微鏡と 1 分子イメージングの用法を掃引高傾斜を構築する方法について説明します。
Tags
Single-molecule Imaging
Extended Field-of-view
Swept Tile Microscope
Piezo Stage
Collimated Light Source
Dichroic Mirror
Tube Lens
Multi-band Pass Filter
Fold-mirror
Objective Lens
Airy Disc
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