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Biochemistry
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单分子跟踪显微镜 - 确定细胞分子扩散状态的工具
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Biochemistry
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JoVE Journal
Biochemistry
Single-Molecule Tracking Microscopy – A Tool for Determining the Diffusive States of Cytosolic Molecules
Please note that all translations are automatically generated.
Click here for the English version.
单分子跟踪显微镜 - 确定细胞分子扩散状态的工具
DOI:
10.3791/59387-v
•
10:21 min
•
September 05, 2019
•
Julian M Rocha
,
Andreas Gahlmann
2
1
Department of Chemistry
,
University of Virginia
,
2
Department of Molecular Physiology & Biological Physics
,
University of Virginia School of Medicine
Chapters
00:04
Title
00:35
Microscope Preparation
02:33
Y. enterocolitica
Culture Preparation
03:33
Data Acquisition
05:54
Data Processing
06:45
Data Post-processing – Overlay of Outlines
07:32
Results: Determining the Diffusive States of YscQ
09:25
Conclusion
Summary
Automatic Translation
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Automatic Translation
3D 单分子定位显微镜用于探测活细菌细胞中荧光标记蛋白质的空间位置和运动轨迹。本文描述的实验和数据分析方案根据集合的单分子轨迹确定细胞蛋白的普遍扩散行为。
Tags
Single-molecule Tracking
3D Tracking
Diffusive States
Cytosolic Molecules
Fluorescence Microscopy
Agarose Pad
Yersinia Enterocolitica
Cell Volume
Z-calibration
MATLAB
HCImage Live
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