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Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
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Biochemistry
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JoVE Journal Biochemistry
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

DOI:
10.3791/56328-v

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08:28 min

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September 19, 2017

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, , , ,
1School of Pharmacy, Tongji Medical College,Huazhong University of Science and Technology, 2Mechanobiology Institute,National University of Singapore, 3Department of Physics,National University of Singapore, 4Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Lab for Soft Functional Materials Research, Department of Physics,Xiamen University, 5Center for Bioimaging Sciences,National University of Singapore

Chapters

  • 00:05Title
  • 01:10Magnetic Tweezers Setup and Identification of Single dsDNA Tether
  • 04:43Single-molecule Manipulation of G4 on the Presence and Absence of Binding Proteins
  • 05:57Results: Single-molecule Manipulation of a G4 and Force-jump Measurements of G4 Unfolding
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

A single-molecule magnetic tweezers platform to manipulate G-quadruplexes is reported, which allows for the study of G4 stability and regulation by various proteins.

Tags

Keywords: G-quadruplexMagnetic TweezersSingle-molecule ManipulationFolding And UnfoldingBinding ProteinsDNA-protein InteractionsHelicase ActivityDNA Tether FormationParamagnetic BeadsNanometer-scale Dynamics

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