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一种分离和扩增人类脂肪衍生的美源性干细胞的无酶方法
JoVE Journal
Medicine
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JoVE Journal Medicine
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
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一种分离和扩增人类脂肪衍生的美源性干细胞的无酶方法

DOI:
10.3791/59419-v

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04:37 min

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December 16, 2019

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, , , ,
1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School,Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Chapters

  • 00:04Title
  • 01:10Isolation of ASCs from Adipose Tissue
  • 02:37Culture of ASCs
  • 03:29Results: An Explant-based Enzyme-free Method of ASCs Isolation
  • 04:02Conclusion

Summary

Automatic Translation

Automatic Translation

该协议提供了一种无酶方法，用于使用外植方法从腹肌成形和脂质酶样本中分离中质性干细胞。没有苛刻的酶或离心步骤提供了临床相关的干细胞，可用于体外研究或转回诊所。

Tags

Adipose-derived Mesenchymal Stem CellsEnzyme-free IsolationExplant MethodCell ExpansionTrypsinizationClinical ApplicationsResearch Applications

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