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一种分离和扩增人类脂肪衍生的美源性干细胞的无酶方法
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
DOI:

04:37 min

December 16, 2019

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Chapters

  • 00:04Title
  • 01:10Isolation of ASCs from Adipose Tissue
  • 02:37Culture of ASCs
  • 03:29Results: An Explant-based Enzyme-free Method of ASCs Isolation
  • 04:02Conclusion

Summary

Automatic Translation

该协议提供了一种无酶方法,用于使用外植方法从腹肌成形和脂质酶样本中分离中质性干细胞。没有苛刻的酶或离心步骤提供了临床相关的干细胞,可用于体外研究或转回诊所。

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