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Developmental Biology
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从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞
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Developmental Biology
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JoVE Journal
Developmental Biology
Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure
Please note that all translations are automatically generated.
Click here for the English version.
从骨骼肌活检程序分离、培养、表征和分化人类肌肉祖细胞
DOI:
10.3791/59580-v
•
00:08 min
•
August 23, 2019
•
Brandon J. Gheller*
1
,
Jamie Blum*
1
,
Sharon Soueid-Baumgarten
,
Erica Bender
,
Benjamin D. Cosgrove
,
Anna Thalacker-Mercer
1
Division of Nutritional Sciences
,
Cornell University
,
2
Meinig School of Biomedical Engineering
,
Cornell University
Chapters
00:04
Title
00:59
Human Muscle Progenitor Cell (hMPC) Isolation
03:11
Imaging Cytometer hMPC Culture Characterization
05:52
Results: Representative hMPC Characterization
07:21
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们介绍从骨骼肌活检组织获得的分离、培养、特征化和区分人类原发性肌肉祖细胞(hMPCs)的技术。
通过这些方法获得和特征化的hMC可用于随后解决与人类肌发生和骨骼肌再生相关的研究问题。
Tags
Muscle Progenitor Cells
Skeletal Muscle Regeneration
Biopsy
Isolation
Culture
Characterization
Differentiation
Pax7
CD56
CD29
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