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Isolamento de células mioepiteliais das glândulas lacrimal e submandibular de Murina adulta
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Myoepithelial Cells from Adult Murine Lacrimal and Submandibular Glands
DOI:

07:15 min

June 11, 2019

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Chapters

  • 00:04Title
  • 00:59Mouse Smooth Muscle Actin (SMA) Cell Labeling and Lacrimal Gland (LG) Harvesting and Microdissection
  • 02:10LG Single-cell Suspension Preparation
  • 04:46Antibody Staining and Fluorescence-activated Cell Sorting (FACS)
  • 05:45Results: Representative MEC and Pericyte Characterization and Isolation
  • 06:38Conclusion

Summary

Automatic Translation

A glândula lacrimal (LG) tem dois tipos de células expressando α-actínio do músculo liso (αSMA): células mioepiteliais (MECs) e pericytes. Os MECS são de origem ectodérmica, encontrados em muitos tecidos glandulares, enquanto os pericitos são células musculares lisas vasculares de origem endodérmica. Este protocolo isola MECS e pericitos de LGS murino.

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