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Scratch migration assay og dorsale Skinfold kammer for in vitro og in vivo analyse af sårheling
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JoVE Journal Medicine
Scratch Migration Assay and Dorsal Skinfold Chamber for In Vitro and In Vivo Analysis of Wound Healing

Scratch migration assay og dorsale Skinfold kammer for in vitro og in vivo analyse af sårheling

DOI:
10.3791/59608-v

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09:34 min

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September 26, 2019

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, , ,
1Institute of Experimental and Clinical Pharmacology and Toxicology, Center for Molecular Signaling (PZMS),Saarland University, 2Institute for Clinical and Experimental Surgery,Saarland University

Chapters

  • 00:04Title
  • 01:05Primary Cell Culture and Small Interfering RNA (siRNA) Transfection
  • 02:56In Vitro Wound Healing (Scratch Migration) Assay
  • 04:13In Vivo Skin Wound Healing Assay
  • 07:11Results: Representative In Vitro and In Vivo Wound Healing
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en protokol for en in vitro scratch assay ved hjælp af primære fibroblaster og for en in vivo hud sårheling analyse i mus. Begge assays er enkle metoder til at vurdere in vitro og in vivo sårheling.

Tags

Scratch Migration AssayDorsal Skinfold ChamberWound HealingIn VitroIn VivoCell MigrationSkin WoundPharmacologically Active CompoundsFibroblastsGenotypeLipid-based Transfection ReagentSiRNACell CultureConfluencyScratch Wound

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