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使用微模式和定量成像绘制哺乳动物细胞的新兴空间组织图
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Mapping the Emergent Spatial Organization of Mammalian Cells using Micropatterns and Quantitative Imaging
Please note that all translations are automatically generated.
Click here for the English version.
使用微模式和定量成像绘制哺乳动物细胞的新兴空间组织图
DOI:
10.3791/59634-v
•
09:56 min
•
April 30, 2019
•
Darren Wisniewski
,
Sally Lowell
,
Guillaume Blin
1
MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences
,
University of Edinburgh
Chapters
00:04
Title
00:51
Micropattern Manufacturing Procedure
02:43
Seeding Procedure
03:44
Fixation
04:32
Immunostaining
05:19
Imaging and Image Analysis
07:55
Results: Representative Micropattern Shape Analyses
09:25
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
这里介绍的方法使用微模式和定量成像来揭示哺乳动物文化内的空间组织。该技术在标准细胞生物学实验室中易于建立,为体外研究模式提供了一个可操作的系统。
Tags
Micropatterns
Quantitative Imaging
Cell Organization
In Vitro Modeling
Cell Patterning
Cell Differentiation
Cell Proliferation
Cell-to-cell Competition
Micropatterning Optimization
Cell Adhesion
Photo Mask
UV Ozone Illumination
Matrix Deposition
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