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利用无脊椎杆菌作为感染模型研究结核分枝杆菌复合物
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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex
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利用无脊椎杆菌作为感染模型研究结核分枝杆菌复合物

DOI:
10.3791/59703-v

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09:23 min

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June 30, 2019

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, , , , , ,
1Section of Pediatric Infectious Diseases and Allergy, Department of Medicine,Imperial College London, 2Department of Pharmacy,National University of Singapore, 3MRC Centre for Molecular Bacteriology and Infection, Department of Medicine,Imperial College London

Chapters

  • 00:04Title
  • 00:59Preparation of M. bovis BCG lux for Infection
  • 03:09Infecting G. mellonella with BCG lux
  • 05:23Measuring the In Vivo Burden of BCG lux in G. mellonella
  • 07:10Results: BCG lux Dose Dependent Virulence
  • 08:31Conclusion

Summary

Automatic Translation

Automatic Translation

最近,肺结核分枝杆菌的可重复、廉价和合乎道德的感染模型被确立为一种可重复、廉价和合乎道德的感染模式。在这里,我们描述和演示了为成功感染生物发光分枝杆菌BCG lux而采取的措施。

Tags

Keywords: Galleria MellonellaTuberculosisInfection ModelMycobacteriumInnate ImmunityAlternative To Mammalian ModelsBioluminescenceBCGCell CultureSample Preparation

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