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JoVE Journal
Genetics

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Transcription Start Site Mapping Using Super-low Input Carrier-CAGE
 

Transcription Start Site Mapping Using Super-low Input Carrier-CAGE

Article DOI: 10.3791/59805-v
June 26th, 2019

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Summary June 26th, 2019

Cap Analysis of Gene Expression (CAGE) is a method for genome-wide quantitative mapping of mRNA 5’ends to capture RNA polymerase II transcription start sites at a single-nucleotide resolution. This work describes a low-input (SLIC-CAGE) protocol for generation of high-quality libraries using nanogram-amounts of total RNA.

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