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1Department of Pharmaceutical Sciences, College of Pharmacy,University of Tennessee Health Science Center, 2Department of Urology,Medical University of South Carolina, 3Department of Urology, College of Medicine,University of Tennessee Health Science Center, 4Department of Pharmacology, College of Medicine,University of Tennessee Health Science Center
Chapters
- 00:04Introduction
- 01:45Dissection and Enzymatic Dissociation of Mucosa-free DSM Pieces
- 03:28Voltage-step Induced Cation Currents from DSM Cells Using Amphotericin-B Perforated Whole-cell Voltage Patch-clamp Technique
- 05:52Results: Investigation of Ion Channel Properties in Isolated DSM Cells
- 07:07Conclusion
Vi beskriver en metode for fremstilling av enkelt nyisolert detrusor glatte muskelceller fra menneskelige urinblæreprøver som bruker en to-trinns enzymatisk prosedyre. De oppnådde levedyktige DSM-cellene kan studeres av ulike enkeltcelleteknikker, inkludert den beskrevne amfotericin-B patch-clamp elektrofysiologi for å avsløre fysiologiske og farmakologiske egenskaper.
