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Journal / Genetics / Transformering, genom-redigering och fenotypning…
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Genetics

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JoVE Journal Genetics
Transformering, genom-redigering och fenotypning av kvävefixerande tropiska Cannabaceae träd Parasponia andersonii
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Transformering, genom-redigering och fenotypning av kvävefixerande tropiska Cannabaceae träd Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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Parasponia andersonii är ett snabbväxande tropiskt träd som tillhör cannabis familjen (Cannabaceae) och kan bilda kvävefixerande rotknölar i samband med Rhizobium. Här beskriver vi ett detaljerat protokoll för omvänd genetiska analyser i P. andersonii baserat på Agrobacterium tumefaciens-medierad stabil transformation och CRISPR/Cas9-baserad genom redigering.

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Genetik Parasponia andersonii Cannabaceae träd trä Agrobacterium tumefaciens stabil omvandling CRISPR-Cas9 genom redigering nodulation Rhizobium mycorrhization
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