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Hastalık Modellerinde Hematopoetik Hücrelerin İncelemi için Lentiviral CRISPR/Cas9-Aracılı Genom Düzenleme
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Lentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models
DOI:

08:14 min

October 03, 2019

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Chapters

  • 00:04Title
  • 00:46Generation and Purification of Lentivirus Particles
  • 03:08Isolation and Transduction of Lineage-negative Cells from Mouse Bone Marrow
  • 05:23Transplantation of Transduced Cells into Lethally Irradiated Mice
  • 05:39Evaluating the Chimerism of Peripheral Blood
  • 06:19Results: Lentiviral Transduction of Mouse Bone Marrow Lineage-negative Cells
  • 07:23Conclusion

Summary

Automatic Translation

Hematopoetik kök ve ata hücrelerinin (HSPC) CRISPR/Cas9 sistemi tarafından hematopoetik sisteme özgü gen modifikasyonları ile fare model sistemlerini hızla geliştirmek için yüksek verimli genom düzenlemesi için protokoller tanımlanmıştır.

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