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抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运
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Neuroscience
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JoVE Journal Neuroscience
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
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抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运

DOI:
10.3791/59982-v

•

08:13 min

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August 02, 2019

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, , ,
1Department of Pharmacology, Feinberg School of Medicine,Northwestern University, 2Department of Cellular Neurophysiology,Institute of Physiology CAS

Chapters

  • 00:04Title
  • 01:13Live Labeling of Surface-expressed Receptors
  • 01:58Surface Expression
  • 02:13Internalization
  • 02:38Recycling
  • 03:17Fixation and Labeling of Fixed Cells
  • 05:14Mounting and Imaging of Samples
  • 05:36Results: Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

本文提出了一种研究分离原发海马培养物的谷氨酸受体(GluR)贩运的方法。使用抗体喂养方法将内源性或过度表达的受体与药理学方法相结合,该方法允许通过调节来识别调节GluR表面表达的分子机制内部化或回收过程。

Tags

Antibody FeedingGlutamate Receptor TraffickingPrimary Hippocampal CulturesSurface ExpressionInternalizationRecyclingParaformaldehyde FixationFab Anti-IgGDynasore

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