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在氧化应激期间细胞粘附和纤维蛋白扩散动力学
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
DOI:

10:57 min

October 13, 2019

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Chapters

  • 00:04Title
  • 01:08Coating Cell Culture Plates with the Extracellular Matrix Protein Fibronectin
  • 02:13Preparing Anchorage-dependent Cells for the Adhesion Assay
  • 05:28Cell Fixation and Antibody Staining for Immunofluorescence
  • 07:41Results: Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
  • 09:43Conclusion

Summary

Automatic Translation

该方法可用于量化细胞粘附的早期动力学,并将锚固依赖细胞扩散到纤维质。此外,此测定可用于研究改变的氧化还原平衡对细胞扩散和/或细胞粘附相关细胞内信号通路的影响。

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