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在氧化应激期间细胞粘附和纤维蛋白扩散动力学
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Biochemistry
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JoVE Journal Biochemistry
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
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在氧化应激期间细胞粘附和纤维蛋白扩散动力学

DOI:
10.3791/59989-v

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10:57 min

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October 13, 2019

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, , ,
1Department of Cell Biology and Physiology,University of North Carolina at Chapel Hill, 2Department of Chemistry,High Point University, 3Biotechnology Program and the Department of Molecular Biomedical Sciences,North Carolina State University

Chapters

  • 00:04Title
  • 01:08Coating Cell Culture Plates with the Extracellular Matrix Protein Fibronectin
  • 02:13Preparing Anchorage-dependent Cells for the Adhesion Assay
  • 05:28Cell Fixation and Antibody Staining for Immunofluorescence
  • 07:41Results: Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
  • 09:43Conclusion

Summary

Automatic Translation

Automatic Translation

该方法可用于量化细胞粘附的早期动力学，并将锚固依赖细胞扩散到纤维质。此外，此测定可用于研究改变的氧化还原平衡对细胞扩散和/或细胞粘附相关细胞内信号通路的影响。

Tags

Cellular AdhesionCell SpreadingEpithelial CellsFibronectinOxidative StressExtracellular MatrixCytoskeletal DynamicsRedox StatusMetastatic CancerAnchorage-dependent CellsCell CultureCell LinesOxidantsBSATrypsin-EDTA

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