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通过直接内切内脂多糖灌输诱导小鼠急性肺损伤
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Medicine
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JoVE Journal Medicine
Inducing Acute Lung Injury in Mice by Direct Intratracheal Lipopolysaccharide Instillation
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通过直接内切内脂多糖灌输诱导小鼠急性肺损伤

DOI:
10.3791/59999-v

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11:07 min

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July 06, 2019

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, , ,
1Department of Anesthesiology and Intensive Care Medicine,University Hospital Bonn

Chapters

  • 00:04Title
  • 00:46Acute Lung Injury Induction
  • 02:56Blood Sampling, Bronchoalveolar Lavage, Organ Harvesting
  • 05:58Tissue Preparation for FACS Analysis
  • 08:57Results: Validation of Murine Acute Lung Injury Model Against Control Animals
  • 10:31Conclusion

Summary

Automatic Translation

Automatic Translation

这里介绍的是一个逐步的过程,通过直接的内切内脂多糖灌输诱导小鼠急性肺损伤,并执行FACS分析血液样本,支气管洗浴液和肺组织。微创性、简单处理、良好的可重复性和疾病严重程度的滴定是这种方法的优点。

Tags

Acute Lung InjuryMouse ModelIntratracheal InstillationLipopolysaccharide (LPS)Animal ResearchLung InflammationReproducible TechniqueMinimally Invasive

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