JoVE Journal
Cancer Research
Cancer Research
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Chapters
Summary
The presence of cancer stem cells have been associated with relapse or poor outcomes after radiotherapy. This manuscript describes the methods to study the radiosensitivity of cancer stem cells in lung cancer cell lines.
Tags
Radiosensitivity Cancer Stem Cells Lung Cancer Cell Lines Radiotherapy Relapse Poor Outcome Radioresistant Stem Cells Putative Markers Flow Cytometry Self-renewal Capacity Sphere Formation Assay Colony Formation Assay Radiosensitivity Study Mechanism Study DNA Damage Repair Reactive Oxygen Species Cell Cycle Arrest Radiation Procedure A549 Cells RPMI-1640 Medium FBS Centimeter Dishes
PLAYLIST
Basic Concepts in Cancer Research
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Education • Core: BiologyCancer -
Education • Cell BiologyAn Introduction to Cell Division -
Education • Core: BiologyEpigenetic Regulation -
Education • Cell BiologyAn Introduction to Cell Motility and Migration -
Education • Cell BiologyAn Introduction to Cell Metabolism -
Research • BiologyUsing Mouse Mammary Tumor Cells to Teach Core Biology Concepts: A Simple Lab Module -
Research • Developmental BiologyInduction and Validation of Cellular Senescence in Primary Human Cells
Cell-Based Assays
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Research • Cancer ResearchCell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry -
Research • Cancer ResearchReal Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells -
Research • BiochemistryAssay for Phosphorylation and Microtubule Binding Along with Localization of Tau Protein in Colorectal Cancer Cells -
Research • Cancer ResearchCounting Proteins in Single Cells with Addressable Droplet Microarrays -
Research • Cancer ResearchSubcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking -
Research • Cancer ResearchLive Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System -
Research • Cancer ResearchA Mimic of the Tumor Microenvironment: A Simple Method for Generating Enriched Cell Populations and Investigating Intercellular Communication -
Research • Developmental BiologyInduction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Capturing Cancer Stem Cells
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Research • Cancer ResearchEstablishment of Cancer Stem Cell Cultures from Human Conventional Osteosarcoma -
Research • Cancer ResearchObtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors -
Research • Cancer ResearchIsolation of Stem-like Cells from 3-Dimensional Spheroid Cultures -
Research • Cancer ResearchIsolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples -
Research • BiologyIsolation of CD133+ Liver Stem Cells for Clonal Expansion -
Research • Cancer ResearchRadiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines
Circulating Tumor Cells and DNA
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Research • Cancer ResearchCharacterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH -
Research • Cancer ResearchSemi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence -
Research • Cancer ResearchMicromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment -
Research • Immunology and InfectionAnalyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow -
Research • Cancer ResearchDetection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids -
Research • Cancer ResearchDetection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients
Mapping Cancer in Spheroids and 3D Culture
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Research • MedicineChick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds -
Research • BioengineeringPreparation and Analysis of In Vitro Three Dimensional Breast Carcinoma Surrogates -
Research • Cancer ResearchMultidimensional Coculture System to Model Lung Squamous Carcinoma Progression -
Research • Cancer ResearchMonitoring Cancer Cell Invasion and T-Cell Cytotoxicity in 3D Culture -
Research • Cancer ResearchAssessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells -
Research • MedicineImaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids -
Research • Cancer ResearchAnalyzing the Communication Between Monocytes and Primary Breast Cancer Cells in an Extracellular Matrix Extract (ECME)-based Three-dimensional System -
Research • Cancer ResearchHow to Study Basement Membrane Stiffness as a Biophysical Trigger in Prostate Cancer and Other Age-related Pathologies or Metabolic Diseases -
Research • Cancer ResearchLongitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography
Research on Animal Models
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Research • Cancer ResearchA Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research -
Research • MedicineDual Bioluminescence Imaging of Tumor Progression and Angiogenesis -
Research • MedicineAn Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy -
Research • Cancer ResearchImmunophenotyping of Orthotopic Homograft (Syngeneic) of Murine Primary KPC Pancreatic Ductal Adenocarcinoma by Flow Cytometry -
Research • Cancer ResearchAn Orthotopic Resectional Mouse Model of Pancreatic Cancer -
Research • Cancer ResearchUtilizing High Resolution Ultrasound to Monitor Tumor Onset and Growth in Genetically Engineered Pancreatic Cancer Models -
Research • MedicineMurine Model for Non-invasive Imaging to Detect and Monitor Ovarian Cancer Recurrence -
Research • Cancer ResearchUsing the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers
Cancer Histology and Pathology
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Research • MedicineDissection of the Mouse Pancreas for Histological Analysis and Metabolic Profiling -
Research • MedicineInstillation and Fixation Methods Useful in Mouse Lung Cancer Research -
Research • MedicineDIPLOMA Approach for Standardized Pathology Assessment of Distal Pancreatectomy Specimens -
Research • Cancer ResearchPerfusion and Inflation of the Mouse Lung for Tumor Histology -
Research • Cancer ResearchElastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue -
Research • MedicineTubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection -
Research • Cancer ResearchUtilizing 18F-FDG PET/CT Imaging and Quantitative Histology to Measure Dynamic Changes in the Glucose Metabolism in Mouse Models of Lung Cancer
Tracking Tumor Metastases
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Research • MedicineExperimental Metastasis Assay -
Research • MedicineModels of Bone Metastasis -
Research • Cancer ResearchCombined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs -
Research • Cancer ResearchAdvanced Animal Model of Colorectal Metastasis in Liver: Imaging Techniques and Properties of Metastatic Clones -
Research • Cancer ResearchAcellular and Cellular Lung Model to Study Tumor Metastasis -
Research • Cancer ResearchA Portal Vein Injection Model to Study Liver Metastasis of Breast Cancer -
Research • Cancer ResearchPathological Analysis of Lung Metastasis Following Lateral Tail-Vein Injection of Tumor Cells -
Research • MedicineQuantitative Analysis of Cancer Metastasis using an Avian Embryo Model -
Research • MedicineGene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Oncogene Profiling
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Research • Cancer ResearchUsing CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells -
Research • BiologyDetecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing -
Research • Cancer ResearchMapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis -
Research • MedicineQuantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors -
Research • MedicineMicroarray-based Identification of Individual HERV Loci Expression: Application to Biomarker Discovery in Prostate Cancer -
Research • Cancer ResearchSequencing Small Non-coding RNA from Formalin-fixed Tissues and Serum-derived Exosomes from Castration-resistant Prostate Cancer Patients -
Research • MedicineProfiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells -
Research • MedicineIdentification of Sleeping Beauty Transposon Insertions in Solid Tumors using Linker-mediated PCR
Investigating Therapies
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Research • MedicineCerenkov Luminescence Imaging (CLI) for Cancer Therapy Monitoring -
Research • Cancer ResearchThe Clinical Application of Tumor Treating Fields Therapy in Glioblastoma -
Research • Cancer ResearchAnticancer Efficacy of Photodynamic Therapy with Lung Cancer-Targeted Nanoparticles -
Research • MedicineProton Therapy Delivery and Its Clinical Application in Select Solid Tumor Malignancies -
Research • ChemistryPolymalic Acid-based Nano Biopolymers for Targeting of Multiple Tumor Markers: An Opportunity for Personalized Medicine? -
Research • MedicineProtocols for Assessing Radiofrequency Interactions with Gold Nanoparticles and Biological Systems for Non-invasive Hyperthermia Cancer Therapy -
Research • Cancer ResearchPreparation of Exosomes for siRNA Delivery to Cancer Cells -
Research • Cancer ResearchAnalysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
