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Journal / Developmental Biology / Dissektion, kultur och analys av primära…
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Developmental Biology

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JoVE Journal Developmental Biology
Dissektion, kultur och analys av primära kraniella neurala krön celler från musen för studiet av neurala Crest cell delaminering och migration
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Dissektion, kultur och analys av primära kraniella neurala krön celler från musen för studiet av neurala Crest cell delaminering och migration

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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Detta protokoll beskriver dissektion och kultur av kranial neurala krön celler från musmodeller, främst för studier av cell migration. Vi beskriver de levande avbildningstekniker som används och analysen av hastighet och cell formförändringar.

Tags

Utvecklingsbiologi cellkultur Live Imaging mus kranial neurala Crest cell migration epitelial-mesenkymala övergång cell motilitet
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