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Medicine
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小鼠肝细胞核球状估计的高通量原位方法
JoVE Journal
Medicine
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JoVE Journal
Medicine
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
Please note that all translations are automatically generated.
Click here for the English version.
小鼠肝细胞核球状估计的高通量原位方法
DOI:
10.3791/60095-v
•
08:44 min
•
April 19, 2020
•
Fátima Manzano-Núñez
,
Ruby Peters
,
Deborah J. Burks
3
,
Luke A. Noon
3
1
Centro de Investigación Príncipe Felipe (CIPF)
,
2
Department of Physiology, Development and Neuroscience
,
University of Cambridge
,
3
Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)
Chapters
00:05
Introduction
01:14
Cyrosectioning
01:54
Fluorescence Immunolabelling
03:55
Fluorescence Image Acquisition and Automated Fluorescence Image Analysis
04:55
Data Analysis
06:24
Results: Representative Murine Hepatocyte Nuclear Ploidy Estimation
08:03
Conclusion
Summary
Automatic Translation
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Automatic Translation
我们提出了一种稳健、经济、灵活的方法,用于测量固定/冷冻组织样本中肝细胞数和核球菌的变化,而不需要流动细胞测量。我们的方法提供了肝细胞学的强大样本范围特征,非常适合跟踪肝损伤和疾病的进展。
Tags
Hepatocyte
Nuclear Ploidy
In Situ Method
High-throughput
Liver Development
Liver Regeneration
Chronic Liver Disease
Nuclear DNA Content
Cryopreserved Liver
Immunolabeling
Ploidy Profiling
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