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小鼠肝细胞核球状估计的高通量原位方法
JoVE Journal
Medicine
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JoVE Journal Medicine
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
DOI:

08:44 min

April 19, 2020

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Chapters

  • 00:05Introduction
  • 01:14Cyrosectioning
  • 01:54Fluorescence Immunolabelling
  • 03:55Fluorescence Image Acquisition and Automated Fluorescence Image Analysis
  • 04:55Data Analysis
  • 06:24Results: Representative Murine Hepatocyte Nuclear Ploidy Estimation
  • 08:03Conclusion

Summary

Automatic Translation

我们提出了一种稳健、经济、灵活的方法,用于测量固定/冷冻组织样本中肝细胞数和核球菌的变化,而不需要流动细胞测量。我们的方法提供了肝细胞学的强大样本范围特征,非常适合跟踪肝损伤和疾病的进展。

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