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Journal
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Biochemistry
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通过低温BN-MS分析进行高分辨率复杂分析
JoVE Journal
Biochemistry
This content is Free Access.
JoVE Journal
Biochemistry
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Please note that all translations are automatically generated.
Click here for the English version.
通过低温BN-MS分析进行高分辨率复杂分析
DOI:
10.3791/60096-v
•
09:33 min
•
October 15, 2019
•
Catrin Swantje Müller
,
Wolfgang Bildl
,
Norbert Klugbauer
,
Alexander Haupt
,
Bernd Fakler
3
,
Uwe Schulte
3,4
1
Institute of Physiology, Faculty of Medicine
,
University of Freiburg, Freiburg, Germany
,
2
Institute of Experimental and Clinical Pharmacology and Toxicology, Faculty of Medicine
,
University of Freiburg, Freiburg, Germany
,
3
Signalling Research Centres BIOSS and CIBSS, Freiburg, Germany
,
4
Logopharm GmbH, Germany
Chapters
00:04
Title
01:16
Preparative BN-PAGE
03:44
Gel Sampling and Digestion
06:32
Results: High-Resolution Complexome Profiling
08:29
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
提出了一种使用微体体进行多功能冷冻BN-MS协议,用于高分辨率复杂体分析。
Tags
CsBN-MS
Protein Complexes
Membrane Proteins
Signal Transduction
Native Protein Complex
Subunit Composition
Gradient Gel
Polymerization
Loading Slots
Running Buffers
Protein Solubilization
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