JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Medicine
/
免疫球蛋白 G N-甘油分析通过超高性能液色谱分析
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Medicine
Immunoglobulin G N-Glycan Analysis by Ultra-Performance Liquid Chromatography
Please note that all translations are automatically generated.
Click here for the English version.
免疫球蛋白 G N-甘油分析通过超高性能液色谱分析
DOI:
10.3791/60104-v
•
11:01 min
•
January 18, 2020
•
Di Liu*
1
,
Xizhu Xu*
2
,
Yuejin Li
,
Jie Zhang
,
Xiaoyu Zhang
,
Qihuan Li
,
Haifeng Hou
,
Dong Li
,
Wei Wang
2,3
,
Youxin Wang
1
Beijing Key Laboratory of Clinical Epidemiology, School of Public Health
,
Capital Medical University
,
2
School of Public Health
,
Shandong First Medical University & Shandong Academy of Medical Sciences
,
3
School of Medical and Health Sciences
,
Edith Cowan University
Chapters
00:05
Title
01:42
Immunoglobulin G (IgG) Isolation
03:57
Glycan Release
05:25
Glycan Labeling and Purification
07:30
Hydrophilic Interaction Chromatography and Analysis of Glycans
08:52
Results: UPLC Chromatogram and Structure
09:30
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
免疫球蛋白G(IgG)N-甘油采用亲水相互作用色谱UPLC的特征是。
此外,IgG
N-
甘油的结构明显分离。这里介绍这个实验方法,以便它可以在研究环境中广泛使用。
Tags
Immunoglobulin G
N-glycan Analysis
Ultra-performance Liquid Chromatography
IgG Purification
Protein G Monolithic Plate
Sample Preparation
Glycan Isomers
Plasma Samples
Glycan Analysis
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
小鼠模型的腔内MCAO脑梗塞评价甲酚紫染色
视网膜脱离模型鼠害由透明质酸钠的视网膜下注射
心率
在小鼠中通过无创M型超声心动图测量
子宫内
人淋巴管内皮细胞中的多参数荧光激活细胞分选分离
VDJ-SEQ:重排免疫球蛋白重链基因的深度测序分析,以揭示的B细胞淋巴瘤克隆演变模式
激光捕获显微切割液相色谱质谱 (LCM-LC/MS) 对肺结核患者药物的空间量化
生物芯片微流控技术在血清过敏源特异性免疫球蛋白 E (sIgE) 检测中的应用
通过在空气-液体界面中暴露培养的人类肺细胞,评估空气中颗粒的急性吸入毒性
通过流式细胞术进行质量控制痰液分析
通过生物电阻抗矢量分析评估流体过载
Read Article