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Journal / Developmental Biology / 전사 요인의 강제 표현에 의한 인간 섬유아세포의 혈생…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
전사 요인의 강제 표현에 의한 인간 섬유아세포의 혈생 재프로그래밍
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Journal (Developmental Biology)
Efficient Gene Transfer in Chick Retinas for Primary Cell Culture Studies: An Ex-ovo Electroporation Approach
Analysis of Epididymal Protein Synthesis and Secretion
Journal (Developmental Biology)
Analysis of Epididymal Protein Synthesis and Secretion
 
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전사 요인의 강제 표현에 의한 인간 섬유아세포의 혈생 재프로그래밍

Article DOI: 10.3791/60112-v • 11:42 min • November 4th, 2019
November 4th, 2019 •
1,2,3, 3, 4, 5,6, 1,2,3
1Molecular Medicine and Gene Therapy, Lund Stem Cell Center, Lund University, 2Wallenberg Center for Molecular, Lund University, 3Center for Neuroscience and Cell Biology, University of Coimbra, 4Skolkovo Institute of Science and Technology, 5Department of Cell, Developmental and Regenerative Biology, Icahn School of Medicine at Mount Sinai, 6Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai

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이 프로토콜은 조혈 줄기 및 전구 세포를 생성하는 전사 인자 GATA2, GFI1B 및 FOS의 발현을 강제함으로써 인간 진피 섬유아세포에서 혈생 프로그램의 유도를 입증한다.

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발달 생물학 문제 153 혈생 재프로그래밍 섬유 아세포 전사 인자 조혈 조혈 줄기 및 전구 세포 내피 - 투 - 조혈 전이
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