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Developmental Biology
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通过转录因子的强制表达,人类纤维细胞的造血再编程
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Hemogenic Reprogramming of Human Fibroblasts by Enforced Expression of Transcription Factors
Please note that all translations are automatically generated.
Click here for the English version.
通过转录因子的强制表达,人类纤维细胞的造血再编程
DOI:
10.3791/60112-v
•
11:42 min
•
November 04, 2019
•
Rita Silvério-Alves
2,3
,
Andreia M. Gomes
,
Ilia Kurochkin
,
Kateri A. Moore
6
,
Carlos-Filipe Pereira
2,3
1
Molecular Medicine and Gene Therapy, Lund Stem Cell Center
,
Lund University
,
2
Wallenberg Center for Molecular
,
Lund University
,
3
Center for Neuroscience and Cell Biology
,
University of Coimbra
,
4
Skolkovo Institute of Science and Technology
,
5
Department of Cell, Developmental and Regenerative Biology
,
Icahn School of Medicine at Mount Sinai
,
6
Black Family Stem Cell Institute
,
Icahn School of Medicine at Mount Sinai
Chapters
00:04
Title
00:41
Lentiviral Production
05:28
Hemogenic Reprogramming
07:34
Optimization of Fibroblast Expansion for ChIP-Seq Analysis at the Onset of Hemogenic Reprogramming
08:59
Results: Hemogenic Fate Induction and Chromatin ChIP-Seq Analysis
10:39
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议通过强制表达转录因子GATA2、GFI1B和FOS来生成造血干细胞和祖细胞,证明在人类皮肤成纤维细胞中诱导造血程序。
Tags
Hemogenic Reprogramming
Human Fibroblasts
Transcription Factors
Lentiviral Production
Fibroblast Transduction
Cell Culture
HEK293T Cells
Transfer Plasmids
Packaging Vector
Envelope Vector
Calcium Chloride
Hematopoietic Stem And Progenitor Cells
Stem Cell Transplantation
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