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Cancer Research
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3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统
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JoVE Journal
Cancer Research
A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies
Please note that all translations are automatically generated.
Click here for the English version.
3D球形模型作为癌症相关纤维细胞分化和入侵体内研究的更多生理系统
DOI:
10.3791/60122-v
•
06:27 min
•
August 08, 2019
•
Ana C. Martins Cavaco
2
,
Johannes A. Eble
1
Institute of Physiological Chemistry and Pathobiochemistry
,
University of Münster
,
2
Instituto de Medicina Molecular, Lisbon
Chapters
00:04
Title
00:41
3D Spheroids as an In Vitro Model for Fibroblast/CAF Differentiation Using Different TGF-β1 Inhibiting Compounds of Cell-matrix Interaction
02:02
Immunofluorescent Staining of Spheroids
04:44
Results: A 3D Spheroid Model for Cancer-Associated Fibroblasts Differentiation and Invasion
05:37
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是建立一个3D体外模型,以研究肿瘤状环境中癌症相关成纤维细胞(CAFs)的分化,可在免疫荧光、转录等不同分析系统中解决。分析和生命细胞成像。
Tags
3D Spheroid Model
Cancer-associated Fibroblasts
Fibroblast Differentiation
Tumor-like Environment
Cell Matrix Interaction
Methylcellulose
Spheroid Formation
TGF-beta
Cell Culture
In Vitro Study
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