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Journal
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Genetics
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通过胚胎移植,生成具有突变倍体肢体的奇美体轴球
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Genetics
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JoVE Journal
Genetics
Generation of Chimeric Axolotls with Mutant Haploid Limbs Through Embryonic Grafting
Please note that all translations are automatically generated.
Click here for the English version.
通过胚胎移植,生成具有突变倍体肢体的奇美体轴球
DOI:
10.3791/60156-v
•
07:17 min
•
January 29, 2020
•
Lucas D. Sanor
,
G. Parker Flowers
,
Craig M. Crews
1
Department of Molecular, Cellular, and Developmental Biology
,
Yale University
Chapters
00:05
Title
00:48
Female Gamete Donor Preparation
01:36
Male Gamete Collection and Preparation
02:51
Female Gamete Collection
03:48
Haploid-Diploid Chimera Generation
05:07
Results: Representative Haploid and Diploid Limb Development
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议的目标是使用胚胎组织移植技术,从Cas9诱变的供体组织中提取单倍体前肢的嵌合体轴片。
Tags
Chimeric Axolotls
Haploid Limbs
Embryonic Grafting
Genetic Screening
Regeneration
Amphibian
In Vitro Fertilization
Gamete Donor
Chorionic Gonadotropin
Spermic Urine
Sperm Irradiation
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