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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

DOI:
10.3791/60191-v

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08:35 min

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December 16, 2019

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, , , ,
1Marine Biological Section, Department of Biology,University of Copenhagen, 2Center for Electromicrobiology,Aarhus University, 3PCO AG, 4Climate Change Cluster,University of Technology Sydney, 5Aarhus University Centre for Water Technology, Section for Microbiology, Department of Bioscience,Aarhus University

Chapters

  • 00:05Title
  • 00:51Planar Oxygen (O2) Optode Fabrication
  • 01:47Rhizo-Sandwich Chamber Preparation
  • 04:01Imaging Setup
  • 04:45Camera Settings and Operation
  • 05:40O2 Optode Calibration and Sample Imaging
  • 06:41Results: Representative Optode Calibration and Lifetime O2 Distribution Imaging
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the use of a novel, frequency-domain luminescence lifetime camera for mapping 2D O2 distributions with optical sensor foils. The camera system and image analysis procedures are described along with the preparation, calibration and application of sensor foils for visualizing the O2 microenvironment in the rhizosphere of aquatic plants.

Tags

Keywords: Luminescence Lifetime ImagingOxygenFrequency-domain-based Camera SystemOptodePlanar Oxygen Sensitive OptodeRhizo-sandwich ChamberOxygen DistributionSpatial And Temporal ResolutionElectron AcceptorBiological SystemsEcologyMedical ResearchBioprintingPressure-sensitive PrintingCocktail OptimizationPET FoilMicroscope SlidesAcrylic-based Instant AdhesiveAir Bubbles

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