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JoVE Journal
Chemistry

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Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System
 

Luminescence Lifetime Imaging of O2 with a Frequency-Domain-Based Camera System

Article doi: 10.3791/60191
December 16th, 2019

Summary December 16th, 2019

We describe the use of a novel, frequency-domain luminescence lifetime camera for mapping 2D O2 distributions with optical sensor foils. The camera system and image analysis procedures are described along with the preparation, calibration and application of sensor foils for visualizing the O2 microenvironment in the rhizosphere of aquatic plants.

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