This content is Free Access.
1Marine Biological Section, Department of Biology,University of Copenhagen, 2Center for Electromicrobiology,Aarhus University, 3PCO AG, 4Climate Change Cluster,University of Technology Sydney, 5Aarhus University Centre for Water Technology, Section for Microbiology, Department of Bioscience,Aarhus University
Chapters
- 00:05Title
- 00:51Planar Oxygen (O2) Optode Fabrication
- 01:47Rhizo-Sandwich Chamber Preparation
- 04:01Imaging Setup
- 04:45Camera Settings and Operation
- 05:40O2 Optode Calibration and Sample Imaging
- 06:41Results: Representative Optode Calibration and Lifetime O2 Distribution Imaging
- 07:46Conclusion
Vi beskriver brugen af et nyt, frekvens-Domain luminescens livstids kamera til kortlægning af 2D O2 -distributioner med optiske sensor folier. Kamerasystemet og billedanalyse procedurerne beskrives sammen med forberedelsen, kalibreringen og anvendelsen af sensor folier til visualisering af O2 -mikromiljøet i rhizosfæren af vandplanter.
Tags
Luminescence Lifetime ImagingOxygenFrequency-domain-based Camera SystemOptodePlanar Oxygen Sensitive OptodeRhizo-sandwich ChamberOxygen DistributionSpatial And Temporal ResolutionElectron AcceptorBiological SystemsEcologyMedical ResearchBioprintingPressure-sensitive PrintingCocktail OptimizationPET FoilMicroscope SlidesAcrylic-based Instant AdhesiveAir Bubbles
