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从新鲜冷冻的小鼠骨骼中产生高质量RNA的激光捕获微解剖协议
JoVE Journal
Biology
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JoVE Journal Biology
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
DOI:

10:11 min

September 16, 2019

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Chapters

  • 00:04Title
  • 01:00Animals and Freeze Embedding
  • 01:41Section Preparation
  • 03:26Rapid Staining Protocol
  • 04:57Laser Capture Microdissection
  • 06:27RNA Extraction
  • 07:43Results: Analysis of RNA Obtained from Fresh-Frozen Mouse Bones
  • 09:10Conclusion

Summary

Automatic Translation

开发了激光捕获微分体(LCM)协议,以获得足够数量的高质量RNA,用于骨细胞的基因表达分析。目前的研究集中在小鼠股骨部分。然而,这里报道的LCM协议可用于研究任何硬组织细胞的基因表达。

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