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从新鲜冷冻的小鼠骨骼中产生高质量RNA的激光捕获微解剖协议
JoVE Journal
Biology
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JoVE Journal
Biology
A Laser Capture Microdissection Protocol That Yields High Quality RNA from Fresh-frozen Mouse Bones
Please note that all translations are automatically generated.
Click here for the English version.
从新鲜冷冻的小鼠骨骼中产生高质量RNA的激光捕获微解剖协议
DOI:
10.3791/60197-v
•
10:11 min
•
September 16, 2019
•
Ana Marek
,
Christiane Schüler
,
María Satué
,
Barbara Haigl
,
Reinhold G. Erben
1
Department of Biomedical Research
,
University of Veterinary Medicine Vienna
Chapters
00:04
Title
01:00
Animals and Freeze Embedding
01:41
Section Preparation
03:26
Rapid Staining Protocol
04:57
Laser Capture Microdissection
06:27
RNA Extraction
07:43
Results: Analysis of RNA Obtained from Fresh-Frozen Mouse Bones
09:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
开发了激光捕获微分体(LCM)协议,以获得足够数量的高质量RNA,用于骨细胞的基因表达分析。目前的研究集中在小鼠股骨部分。然而,这里报道的LCM协议可用于研究任何硬组织细胞的基因表达。
Tags
Laser Capture Microdissection
RNA Extraction
Mouse Bone
Cryosections
Gene Expression Analysis
OCT Compound
Cryostat
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