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可视化和分析星形细胞中细胞内器官和其他货物的细胞内传输
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
Please note that all translations are automatically generated.
Click here for the English version.
可视化和分析星形细胞中细胞内器官和其他货物的细胞内传输
DOI:
10.3791/60230-v
•
00:07 min
•
August 28, 2019
•
Blake A. Creighton*
1
,
Theodore W. Ruffins*
1
,
Damaris N. Lorenzo
1
Department of Cell Biology and Physiology
,
University of North Carolina at Chapel Hill
Chapters
00:04
Title
00:31
Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
02:13
Time-Lapse Imaging
03:03
Image Analysis
05:00
Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
06:38
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这里,我们描述了一种体外活成像方法,用于可视化细胞器的细胞内传输和在小鼠星形细胞中贩运血浆膜蛋白。该协议还提出了一种图像分析方法,以确定货物运输路线和动力学。
Tags
Intracellular Transport
Organelle Motility
Astrocytes
Live Imaging
Fluorescent Labeling
Lipofection
Transfection
Time-lapse Microscopy
Image Analysis
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