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Visualisere og analysere intracellulære transport av organeller og andre cargos i astrocytter
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes
DOI:

00:07 min

August 28, 2019

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Chapters

  • 00:04Title
  • 00:31Fluorescently-Tagged Plasmid Transfection and Fluorescent Probe Late Endosome/Lysosome Labeling
  • 02:13Time-Lapse Imaging
  • 03:03Image Analysis
  • 05:00Results: Representative Quantitative Analyses of Cargo Dynamics in Astrocytes
  • 06:38Conclusion

Summary

Automatic Translation

Her beskriver vi en in vitro Live-imaging metode for å visualisere intracellulære transport av organeller og smugling av plasma membran proteiner i murine astrocytter. Denne protokollen presenterer også en bildeanalyse metodikk for å bestemme laste transport ruter og Kinetics.

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