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Bestemmelse af selv- og inter-(in)kompatibilitetsforhold i abrikos, der kombinerer håndbestøvning, mikroskopi og genetiske analyser
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Determination of Self- and Inter-(in)compatibility Relationships in Apricot Combining Hand-Pollination, Microscopy and Genetic Analyses
DOI:

08:08 min

June 16, 2020

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Chapters

  • 00:04Introduction
  • 00:52Self- and Cross-Pollination in the Laboratory
  • 02:14Evaluating Pollen Viability through In Vitro Pollen Germination
  • 02:30Evaluating Self(in)compatibility by the Observation of Pollen Tube Growth
  • 03:22S-allele Identification
  • 05:40Results: Determination of Self-(in)compatibility and Identification of S-genotype in Apricot Cultivars
  • 06:58Conclusion

Summary

Automatic Translation

Vi præsenterer en metode til at fastsætte bestøvningskravene for abrikos(Prunus armenienca L.) sorter, der kombinerer bestemmelse af selv-(in)kompatibilitet ved fluorescensmikroskopi med identifikation af S-genotype ved PCR-analyse.

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