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小鼠肝脏中细胞类型特异性基因表达分析

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Biology

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Cell Type-specific Gene Expression Profiling in the Mouse Liver

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小鼠肝脏中细胞类型特异性基因表达分析

DOI:

10.3791/60242-v

•

10:06 min

•

September 17, 2019

•

Amber W. Wang, Adam M. Zahm, Kirk J. Wangensteen²

¹Department of Genetics,University of Pennsylvania, ²Department of Medicine,University of Pennsylvania

Chapters

00:04Title
01:21Conjugation of Antibodies to Magnetic Beads
03:36Liver Tissue Lysis
05:33Immunoprecipitation and RNA Isolation
07:37Results: Cell Type-specific Isolation of High-quality RNA by Trap
09:03Conclusion

Summary

Automatic Translation

翻译核糖体亲和性纯化（TRAP）可实现细胞类型特异性翻译mRNA的快速和高效分离。在这里，我们演示了一种将水动力尾静脉注射与肝脏再填充小鼠模型和TRAP相结合的方法，以检查重新填充肝细胞的表达特征。

Tags

TRAP-seq Cell Type-specific Gene Expression Liver Hepatocytes Liver Injury Cell Types Magnetic Beads Protein L Antibody

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