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Celle type-specifik genekspression profilering i muse leveren
JoVE Journal
Biology
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JoVE Journal Biology
Cell Type-specific Gene Expression Profiling in the Mouse Liver
DOI:

10:06 min

September 17, 2019

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Chapters

  • 00:04Title
  • 01:21Conjugation of Antibodies to Magnetic Beads
  • 03:36Liver Tissue Lysis
  • 05:33Immunoprecipitation and RNA Isolation
  • 07:37Results: Cell Type-specific Isolation of High-quality RNA by Trap
  • 09:03Conclusion

Summary

Automatic Translation

Oversættelse af ribosom affinitets rensning (Trap) muliggør hurtig og effektiv isolering af celletype specifik oversættelse af mRNA. Her demonstrerer vi en metode, der kombinerer Hydrodynamisk hale-vene injektion i en musemodel af leveren genindsættelse og fælde for at undersøge udtryks profilen for at genbefolde hepatocytter.

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